Stanford University GCEP are researching E. coli to genetically engineer the microbe to increase the production of fatty acids for the production of biodiesel from biomass. Their three major goals in producing more fatty acids includes increasing the activity of control enzymes that regulate the production of fatty acids, engineering biodiesel biosynthesis and synthesizing tailored fuels. In order to achieve this the researchers plan to increase the carbon flux to fatty acid biosynthesis. Using the genes of other organisms will increase the fermentation process and increase the production of fatty acids. Also looking at the different analogs of fatty acids will help specify fuel sources. Accumulation of glycerol waste can be prevented by decoupling fatty acids biosynthesis. Different analogs of fatty acids can be produced from enzymes in E. coli. The fatty acids can be modified into methyl esters then modified into biodiesel.
Microbial Synthesis -> Biodiesel