Biofuels have been used for sustainable renewable transportation fuels. The main concerns with oil is global warming, land-use, environment concern and rising oil costs. Scientists have been looking at microbes to hope that they could assist in the production of biofuels. They discovered the bacterium Geobacillus thermogluosidasius. This extremophile lives under harsh environments like high temperatures. The bacterium can also survive in high concentrations of ethanol. This microbe has the ability to to ferment C5 and C6 sugars present in cellulosic biomass. Lignocellulose ( sugar and lignin)is present in plants. Yeast have been known to be efficient microbes for biofuel but it lacks to ability to naturally ferment sugars of lignocellulose. The Metabolic flux is the measurement of the enzyme activity that occurs in the metabolic pathway as sugar converts to energy. The researchers used glucose labeled with C-13 to study the distribution of C-13 in the metabolites. This determines the measurement of the metabolic flux and helps researchers determine if a microbe is suitable as biofuels host at very fast time frame. Before this of m technique it took months to determine the metabolic pathway but this technology helps identify the most promising microbes for the production of biofuels at a faster time .
microbes for biofuel