This article talks about how future transformation systems should use hydrogen to make electricity due to its ability to produce renewable and diverse resources. It incourages producing hydrogen through Cell-free Synthetic Pathway Biotransformations(SyPaB). SyPaB assembles sets of stable coenzymes and enzymes to produce en products like hydrogen. The development cycle of SyPaB involves 5 main stages with expert input from many specialists. the first stage is the pathway construction, then enzyme selection, enzyme engineering, enzyme production and purification, and process engineering. Enzyme selection is a critical stage. The extremophile microorganisms are a key resource to the pathway. The enzymes can be engineered to enhance properties (catalytic efficiency selectivity). The SyPaB system can produce an abundant amountof hydrogen from biomass without any carbon emission. The SyPaB system can produces 12 molecules of hydrogen from a single molecule of glucose, which is equivalent of polysaccharides. The reactions from the enzymes produce more energy output in the form of hydrogen than energy input in the form of carbohydrate. The discovery and the use of stable enzymes and coenzymes from extremophile microorganisms (produce hydrogen from low cost renewable sources) could prove revolutionary for the transportation system. The reactions have been enhanced and increased throughout the years by increasing substrates, optimizing enzyme loads, and elevating the reaction temperature to 32C. I obtained this article from The News Magazine of the ASM December 2009 issue.
Extremophile enzymes to generate hydrogen for fuel